Slight cuts in the opening minute to get to the first scene between Kara and her parents quicker
Allura giving her necklace to Kara is removed from the pilot, which causes a continuity error, and also removes the later significance of Kara fiddling with her necklace back at home after facing Vartox
Very tiny cuts to Kara getting her shuttle and launching
Kara's exposition dialogue "My cousin, he didn't need my protection, I didn't have a mission anymore" was rerecorded and sounds more even.
Cat's line "I realise that there are quite a few" when referring to the Tribune employees was cut
Quicker transition between the Kara & Alex scene to Kara's date
Small cut to the cool spinning shot of Kara hovering in the air looking at the plane
Small cuts to the shot of the plane engine on fire, as well as Kara flying after the plane
Pilot's line "We are going down, repeat, we are going down" was cut as well as some short shots around this
Small cuts to the shots of Kara pulling the plane through the bridge
"Supergirl" text added to the title card
Opening credit overlays added to the pilot
Tiny cuts to Alex turning around after saying "What were you thinking?" to Kara
Asian guy saying "Kara, you're a lifesaver! Thanks for doing those comps for me. Cat said I did great work." and Kara replies "Sure Nayoshi, no big deal." is removed
Faster transition between the first office scene and the office roof
The words "to continue your orders and" to Vartox was cut, so the line is just "You are instructed to eliminate the human operatives" instead of "You are instructed to continue your orders and eliminate the human operatives."
The shot of the front of the cars in the car chase is removed
A few frames of Supergirl advancing on the armed robbers is removed
A few frames of Kara and Alex looking at each other after Alex's reveal as a DEO agent removed
A few frames between Kara's lines "I went there... it took me a while" when referring to the bank robbery were removed
Small cuts to shots of Supergirl arriving at the factory
Supergirl's line "What does that have to do with her?" to Vartox is cut
A few frames of Supergirl tackling Vartox cut
The short shot of Vartox advancing on Supergirl after he throws her into the pipes is cut
A small cut before Supergirl says "Did you know about my mom?" to Alex
A few frames of Supergirl leaving Alex at the DEO base cut
A few frames of Supergirl using her x-ray vision to see Alex through the door cut
Cuts between Alex's lines "Made me feel like more / Now / The world needs you to fly, Kara" to quicken the pace
Some frames of Alex getting the Krypton artifact out for Kara cut
About a second of the artifact being turned on removed
Quicker transition between Kara at home and back at the DEO
Supergirl's line "Looks like tonight's the night" when referring to Vartox killing humans cut
Small cuts to Vartox riding in the truck